Immobilization of yeast cell walls with surface displayed laccase from Streptomyces cyaneus within dopamine-alginate beads for dye decolorization

High amounts of toxic textile dyes are released into the environment due to coloring and wastewaters treatment processes' inefficiency. To remove from wastewaters, researchers focused on applying immobilized enzymes mild reaction conditions enzyme nontoxicity. Laccases oxidases with wide substrate specificity, capable degradation many different dye types. Laccase Streptomyces cyaneus was expressed surface Saccharomyces cerevisiae EBY100 cells. The specific activity surface-displayed laccase increased by toluene-induced lysis 3.1 U/g cell walls. For wall immobilization within hydrogel beads, alginate modified dopamine using periodate oxidation reductive amination characterized UV–Vis, FTIR, NMR spectroscopy. Cell dopamine-alginate beads additionally cross-linked oxygen laccase. enzyme's two times higher compared native yield 16 times. decolorized Amido Black 10B, Reactive 5, Evans Blue, Remazol Brilliant Blue 100% efficiency after ten rounds multiple-use retained decolorization 90% 61% Black.